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ACAT1 (K266) polyclonal antibody（KU）

货号: BS3796

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS3796

Product Name :ACAT1 (K266) polyclonal antibody（KU）

Swiss-Prot :P24752

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :ACAT-1 (acetyl-Coenzyme A acetyltransferase 1) is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. Rare defects in the gene encoding ACAT-1 lead to β-ketothiolase deficiency, which is characterized by ketoacidotic attacks. ACAT-2 (acetyl-Coenzyme A acetyltransferase 2) is considered a cytosolic protein and is crucial for cholesterol synthesis. Specifically, both Acetoacetyl-CoA specific thiolases, ACAT-1 and ACAT-2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction, the cleavage of acetoacetyl-CoA into two acetyl-CoA molecules.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ACAT1 (K266) polyclonal antibody detects endogenous levels of ACAT1 protein.

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Acetyl-CoA acetyltransferase, mitochondrial; Acetoacetyl-CoA thiolase; ACAT1; ACAT; MAT; ACAT-1

Immunogen :Synthetic peptide, corresponding to amino acids 235-286 of Human ACAT1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ACAT-1 (K266) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(20ug) Lane2:HepG2 whole cell lysate(20ug) Lane3:The Lung tissue lysate of Rat(20ug) Lane4:The Lung tissue lysate of Mouse(10ug)

Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.