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Adducin β (E497) polyclonal antibody（KU）

货号: BS2523

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS2523

Product Name :Adducin β (E497) polyclonal antibody（KU）

Swiss-Prot :P35612

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Adducins are a family of cytoskeleton proteins encoded by three genes (α, β and γ). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Adducin β (E497) polyclonal antibody detects endogenous levels of Adducin β protein.

BiowMW :~ 110 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; β-Adducin; Adducin1; Adducin-1; β Adducin; Adducin β; Adducin-β

Immunogen :Synthetic peptide, corresponding to amino acids 470-520 of Human Adducin β.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Adducin β (E497) pAb at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:The Brain tissue lysate of Mouse(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Adducin β (E497) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Adducin β (E497) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasm and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.