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ALDH2 monoclonal antibody

货号: MB23512

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23512

Product Name :ALDH2 monoclonal antibody

Swiss-Prot :P05091

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey,Rabbit

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 IF:1/50 - 1/500 FC:1/200 - 1/400

Background :This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms. <br /><br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :56.3kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ALDM; ALDHI; ALDH-E2

Immunogen :Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis usingALDH2 mouse mAb against HepG2(1), A549 (2) cell lysatell and Rat liver(3), Mouse liver (4), Mouse brain (5)tissue lysate.

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALDH2 mouse mAb with DAB staining.

Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescentDNA dve. Red: Actin filaments havebeen labeled with Alexa Fluor-555phalloidin.

Flow cvtometric analvsis ofHeLa cells using ALDH2 mouse mAb(green) and negative control (purple).