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PCNA monoclonal antibody

货号: MB23497

价格：￥ 1200

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB23497

Product Name :PCNA monoclonal antibody

Swiss-Prot :P12004

Host :Mouse

Reactivity :Human,Mouse,Rat,Rabbit,Monkey

Applications :WB,IHC,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 FC:1/200 - 1/400

Background :The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. <br /><br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :28.7kDa

Note :For research use only, not for use in diagnostic procedure.

Immunogen :Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using PCNA mouse mAb with DAB staining.

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）