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His Tag monoclonal antibody

货号: MB23464

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23464

Product Name :His Tag monoclonal antibody

Host :Mouse

Reactivity :Tag

Applications :WB,IP,IHC,IF,FCM

Application_all :WB:1/500-1/1000 IHC:1/100 - 1/1000 IF:1/100 - 1/1000 FC:1/200 - 1/400

Background :The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :6 His tag; HHHHHH tag

Immunogen :Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using His tag mAb against recombinant protein. lane 1 :(100 ng); lane 2 :(50 ng); lane 3 :(25 ng); lane 4 :(10 ng); lane 5 :(2.5 ng); (Expected MW is 50 kDa).