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PDHA1 monoclonal antibody

货号: MB23440

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23440

Product Name :PDHA1 monoclonal antibody

Swiss-Prot :P08559

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/50 - 1/200 FC:1/200 - 1/400

Background :The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :43 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PDHA; PDHAD; PHE1A; PDHCE1A

Immunogen :Purified recombinant fragment of human PDHA1(AA: 241-390) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using PDHA1 mouse mAb against HepG2 (1),Hek293 (2),HL-60 (3),SK-OV-3 (4),PC-3 (5),PANC-1 (6),NRK (7),C2C12 (8), C6 (9), and PC-12 (10) cell lysate.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PDHA1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using PDHA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using PDHA1 mouse mAb (green) and negative control (red).