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RFA2 monoclonal antibody

货号: MB23416

价格：￥ 1200

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB23416

Product Name :RFA2 monoclonal antibody

Swiss-Prot :P15927

Host :Mouse

Reactivity :Human

Applications :WB,IHC,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :29kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RP-A p34, RPA2, REPA2, RPA32, RPA34

Immunogen :Purified recombinant fragment of human RFA2 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using RFA2 mouse mAb against Hela (1), MCF-7 (2), T47D (3), Ramos (4), HEK293 (5), HepG2 (6) and A431 (7) cell lysate.

Immunohistochemical analysis of paraffin-embedded human liver tissues using RFA2 mouse mAb with DAB staining.

免疫沉淀（IP）

免疫组织化学（IHC）