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MCM3 monoclonal antibody

货号: MB23415

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23415

Product Name :MCM3 monoclonal antibody

Swiss-Prot :P25205

Host :Mouse

Reactivity :Human,Rat,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/50 - 1/200 FC:1/200 - 1/400

Background :The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :91kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HCC5; P1.h; RLFB; P1-MCM3

Immunogen :Purified recombinant fragment of human MCM3 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using MCM3 mouse mAb against PC-12 (1), HepG2 (2),Hela (3) and COS7 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using MCM3 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using MCM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using MCM3 mouse mAb (green) and negative control (red).