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H2AFX monoclonal antibody

货号: MB23409

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23409

Product Name :H2AFX monoclonal antibody

Swiss-Prot :P16104

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/50 - 1/200 FC:1/200 - 1/400

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :15.1kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :H2AX; H2A.X; H2A/X

Immunogen :Purified recombinant fragment of human H2AFX expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using H2AFX mouse mAb against HEK293 (1), HepG2 (2), Jurkat (3),Hela (4), Raji (5), K562 (6) and HCT116 (7) cell lysate.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using H2AFX mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using H2AFX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using H2AFX mouse mAb (green) and negative control (red).