产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

NDC80 monoclonal antibody

货号: MB23325

价格：￥ 1200

货期：现货（1-2个工作日）

25ug 50ug 100ug 200ug

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB23325

Product Name :NDC80 monoclonal antibody

Swiss-Prot :O14777

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :74KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80

Immunogen :Purified recombinant fragment of human NDC80 (AA: 443-642) expressed in mammalian cells.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using NDC80 mouse mAb against Hela (1), HepG2 (2), Jurkat (3)and K562 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human mammary cancer tissues using NDC80 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using NDC80 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).