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CDK4 monoclonal antibody

货号: MB23154

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23154

Product Name :CDK4 monoclonal antibody

Swiss-Prot :P11802

Host :Mouse

Reactivity :Human

Applications :IHC,IF,FCM

Application_all :IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :33.7kda

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CMM3; PSK-J3

Immunogen :Purified recombinant fragment of human CDK4 (AA: 77-303) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using CDK4 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using CDK4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)