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ATP1A1 monoclonal antibody

货号: MB23135

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23135

Product Name :ATP1A1 monoclonal antibody

Swiss-Prot :P05023

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :112.8kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CMT2DD; HOMGSMR2

Immunogen :Purified recombinant fragment of human ATP1A1 (AA: 153-288) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using ATP1A1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).