产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

CDH1 monoclonal antibody

货号: MB23085

价格：￥ 1200

货期：现货（1-2个工作日）

25ug 50ug 100ug 200ug

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB23085

Product Name :CDH1 monoclonal antibody

Swiss-Prot :P12830

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :97.5kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324；E-cadherin；E cadherin

Immunogen :Purified recombinant fragment of human CDH1 (AA:extra(155-354)) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CDH1 mouse mAb against PC-3 (1), A431 (2), MCF-7 (3), HT-29 (4), HePG2 (5), C6 (6), A549 (7), NIH/3T3 (8), and C2C12 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using CDH1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using CDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using CDH1 mouse mAb (green) and negative control (red).