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KRT10 monoclonal antibody

货号: MB23054

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23054

Product Name :KRT10 monoclonal antibody

Swiss-Prot :P13645

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :58.8kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BIE; EHK; K10; KPP; BCIE; CK10

Immunogen :Purified recombinant fragment of human KRT10 (AA: 146-455) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using KRT10 mouse mAb against MCF-7 (1), Hela (2), HepG2 (3), T47D (4), HT-29 (5), and A549 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using KRT10 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using KRT10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of A431 cells using KRT10 mouse mAb (green) and negative control (red).