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AURKA monoclonal antibody

货号: MB22365

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22365

Product Name :AURKA monoclonal antibody

Swiss-Prot :O14965

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :45.8kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47

Immunogen :Purified recombinant fragment of human AURKA (AA: 268-404) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using AURKA mouse mAb against HEK293 (1), MCF-7 (2), and Hela (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AURKA mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using AURKA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of HeLa cells using AURKA mouse mAb (green) and negative control (red).