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PRKAA2 monoclonal antibody

货号: MB22314

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22314

Product Name :PRKAA2 monoclonal antibody

Swiss-Prot :P54646

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey,Rabbit

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 IF:1/50 - 1/500 FC:1/200 - 1/400

Background :The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :62.3kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AMPK; AMPK2; PRKAA; AMPKa2

Immunogen :Purified recombinant fragment of human PRKAA2 (AA: 453-552) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using PRKAA2 mouse mAb against HEK293 (1) and COS7 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.

Immunofluorescence analysis of NIH/3T3 cells using PRKAA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow cytometric analysis of Jurkat cells using PRKAA2 mouse mAb (green) and negative control (red).