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ESR1 monoclonal antibody

货号: MB22291

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22291

Product Name :ESR1 monoclonal antibody

Swiss-Prot :P03372

Host :Mouse

Reactivity :Human,Mouse,Rat,Rabbit

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 IF:1/50 - 1/500 FC:1/200 - 1/400

Background :This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :66.2kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ER; ESR; Era; ESRA; ESTRR; NR3A1

Immunogen :Purified recombinant fragment of human ESR1 (AA: 2-185) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ESR1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow cytometric analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).