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UHRF1 monoclonal antibody

货号: MB22264

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22264

Product Name :UHRF1 monoclonal antibody

Swiss-Prot :Q96T88

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :89.8kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95

Immunogen :Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using UHRF1 mouse mAb against MCF-7 (1) and Hela (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using UHRF1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using UHRF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow cytometric analysis of MCF-7 cells using UHRF1 mouse mAb (green) and negative control (red).