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MET monoclonal antibody

货号: MB22237

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22237

Product Name :MET monoclonal antibody

Swiss-Prot :P08581

Host :Mouse

Reactivity :Human,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :155kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HGFR; AUTS9; RCCP2; c-Met; DFNB97

Immunogen :Purified recombinant fragment of human MET (AA: 743-932) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using MET mouse mAb against A549 (1), COS7 (2), Hela (3), HEK293 (4), HepG2 (5), and A431 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using MET mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using MET mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of MCF-7 cells using MET mouse mAb (green) and negative control (red).