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KDM1A monoclonal antibody

货号: MB22175

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22175

Product Name :KDM1A monoclonal antibody

Swiss-Prot :O60341

Host :Mouse

Reactivity :Human,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :93kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AOF2; CPRF; KDM1; LSD1; BHC110

Immunogen :Purified recombinant fragment of human KDM1A (AA: 55-263) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using KDM1A mouse mAb against SK-Br-3 (1), K562 (2), SW480 (3), Jurkat (4), Hela (5), COS7 (6), T47D (7), and HCT116 (8) cell lysate.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using KDM1A mouse mAb with DAB staining.

Immunofluorescence analysis of MCF-7 cells using KDM1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using KDM1A mouse mAb (green) and negative control (red).