产品类别

联系我们

电话:18013895086

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

AKT1 monoclonal antibody

货号: MB22138

价格：￥ 1200

货期：现货（1-2个工作日）

25ug 50ug 100ug 200ug

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB22138

Product Name :AKT1 monoclonal antibody

Swiss-Prot :P31749

Host :Mouse

Reactivity :Human,Rat,Monkey

Applications :WB,IHC,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 FC:1/200 - 1/400

Background :The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. <br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :55.7kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Immunogen :Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using AKT1 mouse mAb against A549 (1), MCF-7 (2), Hela (3), COS7 (4), C6 (5), and HL-60 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using AKT1 mouse mAb with DAB staining.

免疫沉淀（IP）

免疫组织化学（IHC）