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PLCG1 monoclonal antibody

货号: MB22109

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22109

Product Name :PLCG1 monoclonal antibody

Swiss-Prot :P19174

Host :Mouse

Reactivity :Human,Mouse

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :148.5kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1

Immunogen :Purified recombinant fragment of human PLCG1 (AA: 1192-1291) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using PLCG1 mouse mAb against NIH/3T3 (1), Jurkat (2), A431 (3), and Hela (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using PLCG1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using PLCG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Raji cells using PLCG1 mouse mAb (green) and negative control (red).