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MECP2 monoclonal antibody

货号: MB22057

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22057

Product Name :MECP2 monoclonal antibody

Swiss-Prot :P51608

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :52.4kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

Immunogen :Purified recombinant fragment of human MECP2 (AA: 7-148) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using MECP2 mouse mAb against A431 (1) and MCF-7 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using MECP2 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using MECP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using MECP2 mouse mAb (green) and negative control (red).