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HIST2H4A(20Me) monoclonal antibody

货号: MB22040

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22040

Product Name :HIST2H4A(20Me) monoclonal antibody

Swiss-Prot :P62805

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :11.4kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :H4; H4/n; H4F2; H4FN; FO108; HIST2H4

Immunogen :Synthesized peptide of human HIST2H4A ( AA: GGAKRHRK(Me)VLRDNIQ ).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.

Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.

Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow cytometric analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).