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MGMT monoclonal antibody

货号: MB21997

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21997

Product Name :MGMT monoclonal antibody

Swiss-Prot :P16455

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :MGMT (O-6-methylguanine-DNA methyltransferase) is a protein-coding gene. Diseases associated with MGMT include oligoastrocytoma, and spinal cord astrocytoma. GO annotations related to this gene include DNA-methyltransferase activity and calcium ion binding.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :21.6kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EC 2.1.1.63

Immunogen :Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using MGMT mouse mAb against MCF-7 (1), Jurkat (2), HepG2 (3), and PC-3 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using MGMT mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using MGMT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using MGMT mouse mAb (green) and negative control (red).