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PDPK1 monoclonal antibody

货号: MB21970

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21970

Product Name :PDPK1 monoclonal antibody

Swiss-Prot :O15530

Host :Mouse

Reactivity :Human,Mouse,Monkey,Rat

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/50 - 1/200 FC:1/200 - 1/400

Background :Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :63.2kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PDK1; PDPK2; PDPK2P; PRO0461

Immunogen :Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using PDPK1 mouse mAb with DAB staining.

Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red).