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E2F1 monoclonal antibody

货号: MB21634

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21634

Product Name :E2F1 monoclonal antibody

Swiss-Prot :Q01094

Host :Mouse

Reactivity :Human,Mouse

Applications :WB,IHC,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :46.9kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RBP3; E2F-1; RBAP1; RBBP3

Immunogen :Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using E2F1 mouse mAb with DAB staining.

免疫沉淀（IP）

免疫组织化学（IHC）