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SKP1 monoclonal antibody

货号: MB21547

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21547

Product Name :SKP1 monoclonal antibody

Swiss-Prot :P63208

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :19kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L; MGC34403

Immunogen :Purified recombinant fragment of human SKP1 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using SKP1 mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), RAJI (4), PC-12 (5), Cos7 (6), MCF-7 (7) and HepG2 (8) cell lysate.

Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using SKP1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using SKP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of NIH/3T3 cells using SKP1 mouse mAb (green) and negative control (red).