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ACTA2 monoclonal antibody

货号: MB21494

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21494

Product Name :ACTA2 monoclonal antibody

Swiss-Prot :P62736

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :42kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AAT6; ACTSA; α-Smooth Muscle Actin;Alpha-actin-2;Alpha actin 2

Immunogen :Synthesized peptide of human ACTA2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using ACTA2 mouse mAb against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using ACTA2 mouse mAb with DAB staining.

Immunofluorescence analysis of HepG2 cells using ACTA2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).