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PDK1 monoclonal antibody

货号: MB21421

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21421

Product Name :PDK1 monoclonal antibody

Swiss-Prot :Q15118

Host :Mouse

Reactivity :Human,Rat,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. (provided by RefSeq). Tissue specificity: Expressed predominantly in the heart.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :44kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PDK1

Immunogen :Purified recombinant fragment of human PDK1 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.

Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).