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SMAD5 monoclonal antibody

货号: MB21418

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21418

Product Name :SMAD5 monoclonal antibody

Swiss-Prot :Q99717

Host :Mouse

Reactivity :Human,Rat

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :52kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5

Immunogen :Purified recombinant fragment of human SMAD5 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.

Immunohistochemical analysis of paraffin-embedded human brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.

Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).