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SIRT1 monoclonal antibody

货号: MB21361

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21361

Product Name :SIRT1 monoclonal antibody

Swiss-Prot :Q96EB6

Host :Mouse

Reactivity :Human,Mouse,Rat,Rabbit,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :120kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SIR2L1; SIRT1

Immunogen :Purified recombinant fragment of human SIRT1 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysate.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.

Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).