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CDC2 monoclonal antibody

货号: MB21325

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21325

Product Name :CDC2 monoclonal antibody

Swiss-Prot :P06493

Host :Mouse

Reactivity :Human

Applications :WB,IF,FCM

Application_all :WB:1/500 - 1/2000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :34kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222; CDK1

Immunogen :Purified recombinant fragment of CDC2 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CDC2 mouse mAb against Jurkat (1) cell lysate.

Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

免疫沉淀（IP）

免疫组织化学（IHC）