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Pirh2 monoclonal antibody

货号: MB21297

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21297

Product Name :Pirh2 monoclonal antibody

Swiss-Prot :Q96PM5

Host :Mouse

Reactivity :Human,Rat

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :30kDa; 60kDa (homodimer)

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ARNIP; CHIMP; RNF199; RCHY1

Immunogen :Purified recombinant fragment of human Pirh2 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb

Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).