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SMC1 monoclonal antibody

货号: MB21281

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21281

Product Name :SMC1 monoclonal antibody

Swiss-Prot :Q14683

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :143kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; KIAA0178; MGC138332; SMC1alpha; DKFZp686L19178; SMC1A

Immunogen :Purified recombinant fragment of human SMC1 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.

Immunofluorescence analysis of NIH/3T3 cells using SMC1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Hela cells using SMC1 mouse mAb (green) and negative control (purple).