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Cytokeratin 18 monoclonal antibody

货号: MB21183

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21183

Product Name :Cytokeratin 18 monoclonal antibody

Swiss-Prot :P05783

Host :Mouse

Reactivity :Human

Applications :WB,IHC

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000

Background :Cytokeratin 18, also known as CK18, CYK18, KRT18. Entrez Protein NP_000215. It encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :48kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CK18; CYK18; KRT18

Immunogen :Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CK18 mouse mAb against Hela (1), NIH/3T3 (2), A549 (3), Jurkat (4), MCF-7(5), HepG2 (6), A431 (7), HEK293 (8) and K562 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), hepatocarcinoma (B), stomach cancer (C) and colon cancer tissue (D), showing cytoplasmic location with DAB staining using CK18 mouse mAb.