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MonoMethyl-Histone H3-K4 polyclonal antibody

货号: BS7230

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7230

Product Name :MonoMethyl-Histone H3-K4 polyclonal antibody

Swiss-Prot :Q16695

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP, ChIP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:50 - 1:100|ChIP,1:100 - 1:500

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Histone H3 with a site at MonoMethyl-K4 protein.

BiowMW :17KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :H3FT; Histone H3.1t; H3/t; H3t; H3/g

Immunogen :A synthetic monomethylated peptide around K4 of human histone H3(NP_003520.1).

Conjugate :Unconjugated

Modification :Methylated

Western blot analysis of MonoMethyl-Histone H3-K4 expression in Hela (A) whole cell lysates.

Immunofluorescent analysis of MonoMethyl-Histone H3-K4 staining in HEK293T cells.

免疫沉淀（IP）

免疫组织化学（IHC）