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LAMTOR1 polyclonal antibody

货号: BS71562

价格：

一口价 ：￥ 800

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS71562

Product Name :LAMTOR1 polyclonal antibody

Swiss-Prot :Q6IAA8

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC, ELISA

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|ELISA,recommended starting concentration is 1 μg/mL.

Background :As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :LAMTOR1 polyclonal antibody detects endogenous levels of LAMTOR1 protein.

BiowMW :18kDa

Note :For research use only, not for use in diagnostic procedure.

促销价格2 :800

Alternative Name :LAMTOR1;C11orf59;PDRO;Ragulator1;p18;p27RF-Rho

Immunogen :Recombinant protein (or fragment).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates using LAMTOR1 polyclonal antibody at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Confocal immunofluorescence analysis of U2OS cells using LAMTOR1 polyclonal antibody at dilution of 1:100. Blue: DAPI for nuclear staining.