Product Name :SPO11 polyclonal antibody
Application_all :WB (1/500 - 1/1000)
Background :Meiotic recombination protein SPO11: DNA endonuclease, which specifically mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. SPO11 covalently attaches itself to the 5' DNA strand during DSB formation and each strand is cleaved by a composite active site assembled at the interface of two SPO11 monomers, with the two cleavages staggered to produce a two-nucleotide 5' overhang. SPO11 cleavage site selection is driven by a mild sequence bias and a preference for bendable and underwound DNA. Together with TOP6BL, promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Required for the phosphorylation of SMC3, HORMAD1 and HORMAD2
Product :1mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :The antibody was purified by immunogen affinity chromatography.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of SPO11 protein
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Meiotic recombination protein SPO11; Cancer/testis antigen 35; CT35
Immunogen :A synthetic peptide encompassing a sequence within the center region of human SPO11.
Modification :Unmodification
