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FBXO3 polyclonal antibody

货号: BS32507

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS32507

Product Name :FBXO3 polyclonal antibody

Swiss-Prot :Q9UK99

Host :Rabbit

Reactivity :Human

Applications :WB, FC

Application_all :WB (1/500 - 1/1000), FC (1/10 - 1/50)

Background :F-box only protein 3: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, SCF(FBXO3), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins . Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome . In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented . PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation . The SCF(FBXO3) also acts as a regulator of inflammation by mediating ubiquitination and degradation of FBXL2 in response to lipopolysaccharide (LPS) . The SCF(FBXO3) complex specifically recognizes FBXL2 phosphorylated at 'Thr-404' and promotes its ubiquitination

Product :1mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of FBXO3 protein.

BiowMW :~54.6 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FBX3; F-box only protein 3

Immunogen :A synthetic peptide encompassing a sequence within the C-terminal region of human FBXO3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Tyrosinase expression in A375 (A), Podocyte (B), Beas2B (C), PMVEC (D), BV2 (E) whole cell lysates.

Immunohistochemical analysis of Tyrosinase staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.