Western blot analysis of IL-1RA expression in H1792 (A), LO2 (B), THP1 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.
Immunohistochemical analysis of IL-1RA staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).