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AUH polyclonal antibody

货号: BS31133

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS31133

Product Name :AUH polyclonal antibody

Swiss-Prot :Q13825

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/500 - 1/2000), IHC (1/50 - 1/200)

Background :Methylglutaconyl-CoA hydratase, mitochondrial: Catalyzes the fifth step in the leucine degradation pathway, the reversible hydration of 3-methylglutaconyl-CoA (3-MG-CoA) to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) . Can catalyze the reverse reaction but at a much lower rate in vitro . HMG-CoA is then quickly degraded by another enzyme (such as HMG-CoA lyase) to give acetyl-CoA and acetoacetate . Uses other substrates such as (2E)-glutaconyl-CoA efficiently in vitro, and to a lesser extent 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA), crotonyl-CoA ((2E)-butenoyl-CoA) and 3-hydroxybutanoyl-CoA (the missing carboxylate reduces affinity to the active site) . Originally it was identified as an RNA-binding protein as it binds to AU-rich elements (AREs) in vitro . AREs direct rapid RNA degradation and mRNA deadenylation . Might have itaconyl-CoA hydratase activity, converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate...

Product :1mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of AUH protein.

BiowMW :~35.6 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Methylglutaconyl-CoA hydratase mitochondrial; AU-specific RNA-binding enoyl-CoA hydratase; AU-binding protein/enoyl-CoA hydratase

Immunogen :Recombinant fusion protein of human AUH

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of RANBP6 expression in Hela (A), mouse brain (B), rat brain (C), rat testis (D) whole cell lysates.

Immunohistochemical analysis of RANBP6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.