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ACTN1 polyclonal antibody

货号: BS31092

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS31092

Product Name :ACTN1 polyclonal antibody

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/1000 - 1/2000), IHC (1/200 - 1/500)

Background :Alpha-actinin-1: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation

Product :1mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of ACTN1 protein.

BiowMW :~103 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1

Immunogen :Recombinant protein corresponding to mouse ACTN1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of DDX5 (Phospho-Y593) expression in C6 (A), EC9706 (B) whole cell lysates.

Immunohistochemical analysis of DDX5 (Phospho-Y593) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.