Western blot analysis of IFN alpha 1 expression in HEK293T (A), A549 (B), MCF7 (C), mouse lung (D), mouse spleen (E), rat testis (F), rat spleen (G) whole cell lysates.
Immunofluorescent analysis of IFN alpha 1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
