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PKI beta polyclonal antibody

货号: BS31015

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS31015

Product Name :PKI beta polyclonal antibody

Swiss-Prot :Q9C010

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/100 - 1/500)

Background :cAMP-dependent protein kinase inhibitor beta: Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains

Product :1mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of PKI beta protein.

BiowMW :~195 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PRKACN2; cAMP-dependent protein kinase inhibitor beta; PKI-beta

Immunogen :A synthetic peptide encompassing a sequence within the center region of human PKI beta.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Adenosine A3 Receptor expression in MCF7 (A), H1792 (B) whole cell lysates.

Immunofluorescent analysis of Adenosine A3 Receptor staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).