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Prolyl Endopeptidase polyclonal antibody

货号: BS31010

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS31010

Product Name :Prolyl Endopeptidase polyclonal antibody

Swiss-Prot :Q8N6D4

Host :Rabbit

Reactivity :Human

Applications :WB, IHC, IF/ICC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/200)

Background :Prolyl endopeptidase: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has high activity on the succinyl- (suc-) peptide-4-methylcoumaryl-7-amide (MCA) substrates suc-Gly-Pro-Leu-Gly-Pro-MCA, suc-Gly-Pro-MCA and suc-Ala-Ala-Ala-MCA

Product :1mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Prolyl Endopeptidase protein.

BiowMW :~117 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Prolyl endopeptidase; PREP; PE; Post-proline cleaving enzyme; PEP

Immunogen :A synthetic peptide encompassing a sequence within the N-term region of human Prolyl Endopeptidase.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of ALDOA expression in MCF7 (A), Hela (B), NIH/3T3 (C) whole cell lysates.

Immunohistochemical analysis of ALDOA staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.