Product Name :VSV-G-Tag Mouse monoclonal antibody
Application_all :WB: 1:5000~1:10000
IF: 1:200
Background :Epitope tagging is a widely accepted technique that involves the fusion of an epitope labeled peptide to a certain protein as a marker for gene expression. This technique allows gene expression to be easily monitored during western blotting, immunoprecipitation and immunofluorescence by using an antibody that recognizes such an epitope. Amino acid sequences that are widely used for epitope tagging are as follows; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which correspond to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product, and Vesicular stomatitis virus glycoprotein, respectively.
Product :Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :Affinity-chromatography
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :VSV-G-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal VSV-G- tagged proteins.
Figure1 :IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate,WB 1:5000
1.untransfected 293 cell lysate
2.transfected 293 cell lysate with VSV-G-tag fusion protein
3.IP(untransfted 293+anti-VSV-G mAb+Protein G agarose)
4.IP (transfected 293+ normal Mouse IgG+Pr
Note :For research use only, not for use in diagnostic procedure.
Immunogen :Synthetic peptide VSV-G-Tag (YTDIEMNRLGK).
Modification :Unmodification
