Product Name :His-Tag mouse monoclonal antibody-HRP
Application_all :WB: 1:2000~1:20000
Background :The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.
Product :0.5mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :His-tag mAb-HRP detects over-expressed or recombinant proteins containing the 6×His epitope tag.
Figure1 :Western blot (WB) analysis of His-tag mAb-HRP at 1:2000 dillution
Lane1:HEK293F whole cell lysate
Lane2:HEK293F whole cell lysate,transfected (C-His).
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Immunogen :Synthetic peptide His-tag.
