Product Name :Flag-Tag mouse monoclonal antibody-HRP
Application_all :WB: 1:2000~1:20000
Background :Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
Product :0.5mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Flag-Tag mAb-HRP detects over-expressed or recombinant proteins containing the Flag epitope tag.
Figure1 :Western blot (WB) analysis of Flag-Tag mAb-HRP at 1:2000 dillution
Lane1:HEK293F whole cell lysate ,untransfected
Lane2:HEK293F whole cell lysate , transfected (M-Flag)
Lane3:HEK293F whole cell lysate , transfected (N-Flag)
Lane4:HEK293F whole cell lysate , transfected (C-Flag)
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK
Immunogen :Synthetic peptide Flag-tag.
