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β-Actin (I102) polyclonal antibody

货号: AP0060
价格 400
货期 现货(1-2个工作日)
25ug 50ug 100ug 200ug
No. :AP0060
Product Name :β-Actin (I102) polyclonal antibody
Host :Rabbit
Reactivity :Human,Mouse,Rat,zebrafish,Odontobutis obscurus
Applications :WB IP IF
Swiss-Prot :N/A
Application_all :WB:1:5000~1:20000 IP: 1:100~500 IF: 1:500~1000
Background :All eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :β-actin (I102) polyclonal antibody detects endogenous levels of β-actin protein.
Figure3 :Immunofluorescence analysis of RAW264.7 cells using β-actin (I102) pAb at dilution of 1:500 (40x lens).
Figure4 :Immunoprecipitation of MCF-7 cell lysates using β-actin (I102) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4and lane 5) .Lane 1 is 30% input. The western blot was probed using β-Actin pAb.
BiowMW :~ 42 kDa
Note :For research use only, not for use in diagnostic procedure.
Pathway :Contribution of ECM and Cytoskeletal Factors to EMT,Adherens Junction signaling,
Alternative Name :Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β; Beta actin;
Immunogen :Recombinant full length Human β-Actin.
Conjugate :Unconjugated
Modification :Unmodified
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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com